Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
Medchemexpress LLC L-lysyl-L-cysteine trifluoroacetate | 97.8% | 477.38 g/mol | C13H21F6N3O7S | 5 MG
L-lysyl-L-cysteine trifluoroacetate is a dipeptide formed from lysine and cysteine, provided as a solid TFA salt for research use. The material is characterized by a reported purity, defined molecular formula and molecular weight, and specified storage recommendations for powder and solutions.
- Dipeptide composed of lysine and cysteine.
- Supplied as trifluoroacetate (TFA) salt in solid powder form.
- Reported purity 97.8%.
- Molecular formula C13H21F6N3O7S and molecular weight 477.38 g/mol.
- Available in small research quantities: 5 mg, 10 mg, 25 mg.
- Store sealed, away from moisture; powder: -80°C for 2 years, -20°C for 1 year; in solvent: -80°C for 6 months, -20°C for 1 month.
Medchemexpress LLC Tam557 Tfa | 98.5% | 1119.34 | 5 MG
TAM557 TFA is a cytotoxic tubulysin compound modified for conjugation to transport vehicles such as proteins, peptides, small molecules, or polymeric carriers that can carry a targeting principle. It is for research use only and not sold to patients.
- Cytotoxic tubulysin compound
- Modified for conjugation to transport vehicles
- Available in 1 mg, 5 mg, 10 mg, and 50 mg sizes
Medchemexpress LLC WAAG-3R TFA | 99.8% | 1644.74 | 1 MG
WAAG-3R TFA is a biologically active peptide. Aggrecanases, which are part of the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases, cleave aggrecan, a main structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a significant aggrecanase in human osteoarthritic cartilage. This FRET peptide was utilized in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay with Ex/Em = 340/420 nm.
- Biologically active peptide.
- Utilized in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay.
- FRET peptide with Ex/Em = 340/420 nm.
Medchemexpress LLC Lys-CoA TFA (Lys-coenzyme A) | 98.5% | 1 MG
Lys-CoA TFA is a selective p300 histone acetyltransferase (HAT) inhibitor with an IC50 of 50-500 nM. It demonstrates over 100-fold selectivity for p300 compared to PCAF (IC50=200 μM). This compound works by inhibiting p300 HAT activity-dependent transcriptional activation.
- Selective p300 histone acetyltransferase (HAT) inhibitor
- IC50 of 50-500 nM for p300
- Over 100-fold selectivity for p300 compared to PCAF
- Induces a senescent phenotype in human normal melanocytes at 0.1 and 1 mM concentrations
- Inhibits cyclin E expression in a dose-dependent manner
Medchemexpress LLC Apstatin TFA | 99.4% | 25 MG
Apstatin TFA is a potent aminopeptidase P (APP) inhibitor. It exhibits Ki values of 2.6 μM for rat APP and 0.64 μM for human APP. This compound has also demonstrated cardioprotective effects.
- Potent aminopeptidase P (APP) inhibitor
- Effective at 2.6 μM for rat APP
- Effective at 0.64 μM for human APP
- Shows cardioprotection
- Reduces myocardial infarct size in acute myocardial ischemia models
- For research use only
Strem, An Ascensus Company CAS# 76-05-1. 500g. Trifluoroacetic acid, 99%. MFCD00004169
CAS# 76-05-1. 500g. Trifluoroacetic acid, 99%. MFCD00004169. Molecular Weight: 114.02. Molecular Formula: CF3COOH. Color/form: colorless liq. Strem# 09-7160. http://www.strem.com/catalog/v/09-7160/
Medchemexpress LLC 3x DYKDDDDK Tag (TFA) | 98.17% | 3116.90 | 5 MG
DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins when antibodies are not available. It is often used in a 3X FLAG format for purifying difficult proteins that accumulate in low abundance.
- Useful tool for investigating protein function and localization
- Often used in 3X FLAG format for purifying difficult proteins
- Suitable for proteins that accumulate in low abundance
Medchemexpress LLC TfR-T12 TFA | 99.1% | 1604.75 | 1 MG
TfR-T12 TFA is a BBB-penetrated transferrin receptor (TfR) binding peptide. It binds to the TfR and is subsequently internalized into TfR-expressing cells, making it suitable for research applications.
- Displays a binding affinity in the nM range.
- Synthetic peptide obtained by phage display.
- Binds to a different site on TfRs compared with transferrin.
- Can be chemically conjugated to construct multifunctional lipid vesicles.
Medchemexpress LLC Alvespimycin (17-DMAG) trifluoroacetate | 00-00-0 | MFCD08457919 | 99.0% | 730.77 g/mol | C34H49F3N4O10 | 5 MG
Alvespimycin TFA is the trifluoroacetate salt of 17-DMAG, a small-molecule inhibitor of heat shock protein 90 (Hsp90) used as a research reagent to study Hsp90-dependent signaling and anticancer mechanisms. The compound binds Hsp90 with an EC50 of 62 nM and is supplied as a high-purity, pale purple solid for laboratory use.
- Potent Hsp90 inhibitor (EC50 62 nM).
- Trifluoroacetate (TFA) salt form for consistent handling.
- High purity (reported ~98.97%).
- Molecular weight 730.77 g/mol.
- Pale purple solid appearance.
- Storage: sealed, away from moisture and light; in solvent, -80°C (6 months) or -20°C (1 month).
Medchemexpress LLC Bio-AMS TFA | 1393881-52-1 | 98.0% | 685.65 | 1 MG
Bio-AMS (TFA) is a potent bacterial biotin protein ligase inhibitor. It exhibits selective activity against *Mycobacterium tuberculosis* (Mtb) and hinders fatty acid and lipid biosynthesis. It is for research use only and not sold to patients.
- Potent bacterial biotin protein ligase inhibitor.
- Selective activity against *Mycobacterium tuberculosis* (Mtb).
- Arrests fatty acid and lipid biosynthesis.
- Excellent antitubercular activity against Mtb H37Rv and MDR/XDR-TB strains with MICs ranging from 0.16 to 0.625 μM.
- Its activity is not affected by changes to the primary carbon source.
- Inhibits growth of Mtb in Mtb-infected mouse macrophages in a concentration-dependent manner (2.5, 5 and 10 μM; 24 h).
- Shows no signs of mitochondrial toxicity.
Medchemexpress LLC Cl2e-SN-38 TFA | 99.1% | 1855.95 | C89H117F3N14O26 | 25 MG
CL2E-SN38 TFA is a trifluoroacetate salt of an antibody-linked SN-38 conjugate used for antibody-drug conjugate (ADC) research. It acts as a topoisomerase I inhibitor and is supplied as a high-purity solid characterized for in vitro and in vivo formulation.
- Purity: 99.07% (reported on product page)
- Form: trifluoroacetate salt
- Molecular weight: 1855.95
- Chemical formula: C89H117F3N14O26
- Solubility: soluble in DMSO (example: 100 mg/mL); in vivo formulations reported at ≥2.5 mg/mL
- Storage: protect from light; store under nitrogen; in solvent: -80°C for long term
- Intended use: research reagent for ADC development and topoisomerase I inhibition studies
Medchemexpress LLC VTP-27999 (TFA) | 1013937-63-7 | 97.0% | C28H42ClF3N4O7 | 5 MG
VTP-27999 (TFA) is an alkyl amine Renin inhibitor useful for hypertension and end-organ diseases. This product is for research use only.
- Alkyl amine Renin inhibitor
- Useful for hypertension research
- Useful for end-organ diseases research
- For research use only
Medchemexpress LLC TP-5801 TFA | 99.6% | 641.48 | 5 MG
TP-5801 TFA is an orally active TNK1 (non-receptor tyrosine kinase) inhibitor with anti-tumor activity.
- TNK1 inhibitor with an IC50 of 1.40 nM.
- Inhibits TNK1-driven, BCR-ABL-driven and IL-3-driven Ba/F3 cell growth.
- Inhibits TNK1-dependent L540 cell growth.
- Demonstrates efficacy in mouse survival models.
- Capable of inhibiting localized tumor growth in vivo.
- For research use only.
Medchemexpress LLC Gallinamide A TFA | 1352920-57-0 | 706.79 | 1 MG
Gallinamide A TFA is a linearly depositing peptide and a potent inhibitor of cathepsin L (CatL) with an IC50 of 17.6 pM. It inhibits SARS-CoV-2 infection by inhibiting CatL (EC50: 28 nM) and also inhibits Plasmodium falciparum (IC50: 50 nM). It is for research use only and not sold to patients.
- Inhibits cathepsin L, B, V, K, and S.
- Effective against SARS-CoV-2 infection.
- Inhibits P. falciparum.
- Exhibits low cytotoxicity in VeroE6 cells.
- Shows synergistic effects with TMPRSS2 inhibitor Nafamostat.